MOAMR – One Health
01/12/2020 – 30/11/2023
One Health Approach – recognizes that human health is linked to animal health and the environment. The main reason for the development of ‘One Health Approach’ was the emerging problem of the antimicrobial resistance to almost all available antibiotics and the rapid spreading of the resistance genes to almost all bacterial populations. Aquatic ecosystems (waters and waste waters) and animals are known to play an important role as reservoir and carrier for a number of resistance genes. It is recently established that resistant bacteria are no longer restricted to hospitals, but resistance genes and bacterial strains are also dispersed through various environmental routes. Escherichia coli a well-established Feacal Indicator Bacterium, has been linked to the onset of waterborne outbreaks, is considered as the reference bacterium for applying population genetic techniques and in ‘real life’ is the most common Gram-negative pathogen isolated from gastroenteritis in developing countries. Thus, the bacterium has been chosen in order to determine the diversity of antibiotic resistance patterns and the genotypes of circulating various aquatic habitats during the doctoral thesis. The experimental procedure includes 1) antibiotic susceptibility testing through disks diffusion assays, 2) PCR amplification of antibiotic resistance genes (ARG), 3) molecular typing implementing PFGE andMLST techniques, 4) estimation of the transmission frequency of plasmid-born genes via conjugation assay, 5) plasmid analysis via PCR-based replicon typing (PBRT) and S1-PFGE techniques. Finally a subset of isolates which fulfill certain specific criteriawill be undergone for further genetic analysis applying advanced sequence basedmethodologies (Next Generation and Whole Genome Sequencing).
Special Account for Research Grants – Uniwa
€ 36,000
https://bisc.uniwa.gr/profile/dioli-chrysoyla/
